SYBR Green real-time reverse transcription-polymerase chain reaction assay for the generic detection of coronaviruses
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چکیده
منابع مشابه
Diagnosis of Foot-and-Mouth Disease Virus by Real Time Reverse Transcription Polymerase Chain Reaction Assay in Iran
Background and Aims: Accurate and rapid diagnosis is necessary for effective control and prevention of foot-and-mouth disease (FMD). In present study, was evaluated real time reverse transcription-polymerase chain reaction (rRT-PCR) assay along with diagnostic routine methods for the detection of all seven serotypes of FMD virus (FMDV), namely O, C, A, SAT1, 2, 3 and Asia 1 in biological sample...
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The definitive diagnosis of lyssavirus infection (including rabies) in animals and humans is based on laboratory confirmation. The reference techniques for post-mortem rabies diagnosis are still based on direct immunofluorescence and virus isolation, but molecular techniques, such as polymerase chain reaction (PCR) based methods, are increasingly being used and now constitute the principal tool...
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Polymerase chain reaction (PCR)-based assays can target either DNA (the genome) or RNA (the transcriptome). Targeting the genome generates robust data that are informative and, most importantly, generally applicable. This is because the information contained within the genome is context-independent; i.e., generally, every normal cell contains the same DNA sequence--the same mutations and polymo...
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reaction (RT-PCR) assay was developed to rapidly detect the severe acute respiratory syndrome–associated coronavirus (SARS-CoV). The assay, based on multiple primer and probe sets located in different regions of the SARSCoV genome, could discriminate SARS-CoV from other human and animal coronaviruses with a potential detection limit of <10 genomic copies per reaction. The real-time RTPCR assay ...
متن کاملdiagnosis of foot-and-mouth disease virus by real time reverse transcription polymerase chain reaction assay in iran
background and aims: accurate and rapid diagnosis is necessary for effective control and prevention of foot-and-mouth disease (fmd). in present study, was evaluated real time reverse transcription-polymerase chain reaction (rrt-pcr) assay along with diagnostic routine methods for the detection of all seven serotypes of fmd virus (fmdv), namely o, c, a, sat1, 2, 3 and asia 1 in biological sample...
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ژورنال
عنوان ژورنال: Archives of Virology
سال: 2006
ISSN: 0304-8608,1432-8798
DOI: 10.1007/s00705-006-0840-x